Manual

Product Number: PCK34

Shipping and Storage

Stored at -20℃, valid for 12 months.

Component

Description

This kit is specifically designed for one-step RT-PCR experiments, and can be used to conveniently and quickly complete reverse transcription and PCR amplification reactions in the same reaction tube. During the reaction process, there is no need to open the tube cap to add reagents, which avoids contamination while improving detection sensitivity and experimental efficiency. This kit includes the OneStep Enzyme Mix optimized for optimal ratio (including mutated TRUEscript M-MuLV H Minus reverse transcriptase, hot start HotMaster Taq DNA polymerase, and RNasin inhibitor mix), as well as a unique 2 × RT-PCR Mix reaction system suitable for reverse transcription and PCR amplification. The RNase H activity of this enzyme M-MuLV (RNase H ˉ) is deficient. Compared with M-MuLV, it has stronger extensibility and stability, and can be used for longer cDNA synthesis and the construction of high proportion full-length cDNA libraries. At the same time, the enzyme enhances heat resistance and can reverse transcribe at 42-50 ° C, improving the efficiency of complex secondary structures and GC rich template reverse transcription.

Application

Suitable for high copy and low copy gene testing; RNA templates with high GC content or complex secondary structures.