Manual

Product Number: PCK59

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This kit is a specialized reagent for Real Time PCR using SYBR Green I intercalating fluorescence. When used for Real Time qRT-PCR, it enables continuous amplification within the same reaction tube without the need to open the tube for reagent addition, allowing real-time detection of amplified products. This eliminates contamination while improving detection sensitivity and experimental efficiency, making it highly suitable for detecting trace RNA, especially RNA viruses.  The kit includes an optimized OneStep Enzyme Mix (containing mutated TRUEscript M-MuLV H Minus reverse transcriptase, hot-start HotMaster Taq DNA polymerase, and RNasin inhibitor mix), as well as a unique 2×qRT-PCR SybrGreen Mix system suitable for reverse transcription and fluorescent PCR amplification. The M-MuLV (RNase H⁻) enzyme lacks RNase H activity, offering stronger extension capability and stability compared to M-MuLV. Additionally, this enzyme enhances heat resistance, enabling reverse transcription at 42-50°C and improving reverse transcription efficiency for templates with complex secondary structures or high GC content.  The inclusion of high-quality hot-start enzyme HotMaster Taq DNA Polymerase minimizes nonspecific amplification products throughout the PCR process, significantly improving the accuracy of fluorescent quantitative PCR. This product yields excellent standard curves across a wide quantitative range, enabling precise quantification of target genes with good repeatability and high reliability.

Application

Suitable for high copy and low copy gene testing; RNA templates with high GC content or complex secondary structures.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).