Manual

Product Number: PCM18

Shipping and Storage

For long-term storage, please store in the dark at -20℃. After thawing, it can be stably stored for one month at 4℃ in the dark, avoiding repeated freezing and thawing as much as possible.

Component

Description

Universal Visual SYBR qPCR Master Mix is a specialized qPCR reagent for SYBR Green I chimeric dye method. It is a 2 × premix containing all qPCR components except primers and DNA samples, which can reduce operation steps, shorten sample addition time, and lower the risk of contamination. Its core component is antibody modified hot start Taq DNA polymerase, combined with carefully optimized buffer system and PCR reaction promoter, which has strong specificity and high amplification efficiency, and can effectively inhibit non-specific amplification. It can accurately quantify templates with a wide concentration range and obtain stable and reliable qPCR results. This product already contains universal calibration dyes and is compatible with the vast majority of qPCR devices, without the need for additional dyes to calibrate the instrument.

This product can utilize the color changing effect generated by mixing different dyes to track the pipetting process, thereby significantly reducing pipetting errors. The Universal Visual SYBR qPCR Master Mix contains blue dye, while the 10 × Dilution Buffer contains yellow dye. When a template diluted with Dilution Buffer (yellow) is added to the Universal Visual SYBR qPCR Master Mix (blue), a color change effect from blue to green occurs, allowing for accurate determination of whether the template has been added based on the liquid color.