Manual

Shipping and Storage

Ice pack transportation; Stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Component

Description

Exonuclease I is derived from the recombinant expression of the Exo I gene in E. coli strains. This enzyme has the activity of hydrolyzing single stranded DNA from the 3-5 “direction, gradually releasing deoxyribonucleic acid 5” monophosphate and leaving intact 5 “terminal dinucleotides. This product is mainly used for the degradation and digestion of primers after PCR amplification, and is inactive for double stranded DNA and 3OH terminal DNA chains blocked by phosphoryl or acetyl groups.

Application

Commonly used to remove single stranded primers in PCR reactions before Sanger sequencing or SNP analysis; Remove single stranded primers from nested PCR; Purification of PCR products by enzymatic method; Remove single stranded DNA containing 3 ‘hydroxyl terminus from nucleic acid mixtures.

Specification

1. Source: Escherichia coli strain carrying the exonuclease I gene cloned from E. coli NM554.
2. Enzyme storage solution: 10mM Tris HCl, 100mM NaCl, 5mM β – ME, 0.5mM EDTA, 100µg/ml BSA, 50% Glycerol pH 7.5 @ 25℃.
3. Unit definition: 1 unit refers to the amount of enzyme required to catalyze the release of 10nmol of acid soluble nucleosides within 30 minutes at 37℃.
4. Inactivation or inhibition: react at 80℃ for 20 minutes.