Manual

Product Number: RE05881

Shipping and Storage

Store at -20℃ for 2 years.

Components

Description

Nt.BbvCI is a type of endonuclease that cleaves only one strand of dsDNA substrate; Create incisions on the dsDNA substrate without incising the dsDNA. Nt.BbvCI is commonly used for nucleic acid isothermal amplification (such as SDA, RCA), where Nt.BbvCI generates DNA gaps that trigger polymerase chain displacement reactions, repeating cutting, displacement, and extension processes to achieve nucleic acid index amplification.

Nt.BbvCI in Universal FlashCut™ or FlashCut™ Color buffers have excellent activity. FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. FlashCut™ The migration rate of red dye of Color Buffer and 2500bp double stranded DNA fragment in 1% agarose gel is close to each other; The migration rate of yellow dye and 10bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1×FlashCut™ Buffer.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Definition of Activity

The active unit (U) refers to the amount of enzyme required to completely convert 1µg of supercoiled p615 DNA into an open-loop form within 1 hour at 37 ℃ in a 50μL reaction system.

Quality control

1. Functional activity testing: at 37℃, in 20μL of universal FlashCut™ In the reaction system, 5U Nt.BbvCI can convert 1μg p615 into an open-loop form within 15 minutes.
2. Ultra long incubation test: incubate 5U Nt. BbvCI and super spiral p615 DNA substrate at 37℃ for 16 h, and detect no change of open loop DNA by agarose gel electrophoresis.