2× pfu PCR Master Mix
2024-10-28
2× QuickPro PCR Master Mix
2024-10-28
Product Number: EN01
Shipping and Storage
-20°C.
Components
| Component | EN01 4000U | EN01 20000U |
| Exonuclease, 20 U/μl | 200μl | 5×200μl |
| 10×Exo I Reacting Buffer | 1mL | 5×1mL |
Description
This product is derived from recombinant E.coli strain, carrying the Exo I gene, with exonuclease activity of hydrolyzing single-stranded DNA from the 3'-5' direction, which can gradually release deoxyribonucleic acid 5 'monophosphate and leave a complete 5' end dinucleotide. This product is mainly used to degrade digestion primers after PCR amplification, and has no activity for double-stranded DNA and 3 'OH terminal DNA strands closed by phosphoryl or acetyl groups.
Unit definition
The amount of enzyme required to catalyze the release of 10 nmol of soluble nucleotide within 30 minutes at 37°C was defined as 1 unit of activity (U).
Quality Control
The purity was higher than 95% after SDS-PAGE electrophoresis and Coomassie bright blue staining. The addition of BSA can ensure the stability of enzyme.