Manual

Product Number: TR3012

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-LV Transmission Kit is an enhancer developed and synthesized by our company for chronic viral infections, which is synthesized using nanotechnology. The EZ-LV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-LV Transmission kit significantly improves the efficiency of lentiviral infection while minimizing cell toxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-LV Transmission kit for enhancement, the cell fusion degree during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the cell fusion degree is around 50% (adjust the suspension cells as needed, do not use too high cell density).
2. Before using the EZ-LV Transmission kit enhancer, the optimal MOI value (Multiplicity of Infection) for infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the expression level of the target protein. For cells with active division, such as Hela and 293 cells, when MOI=1-3, more than 80% of cells express the target gene. For non dividing cells, such as primary cells, the infection efficiency is low, and MOI gradient experiments are needed to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ-LV Transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ-LV Transmission kit and infection efficiency. Under other fixed conditions, the dosage of enhancer is within the range of 1-3 times the recommended dosage. The larger the dosage, the higher the infection efficiency.

Other related reagents

TR3012: Chronic virus infection enhancement reagent.

TR3003: Adenovirus infection enhancing reagent.

TR3041: Retroviral infection enhancing reagent.

TR3015: Virus infection enhancing reagent in animal bodies.