Product Number: RT5060
Shipping and Storage
Store at -20℃.
Components
| Component | RT5060 200KU |
| ThermoPlus M-MLV Reverse Transcriptase (RNase H-) | 1 ml |
| 5×RT Buffer | 1 ml |
Description
ThermoPlus M-MLV Reverse Transcriptase(RNase H-),encoded by Moloney Murine Leukemia Virus (M-MLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. thermol plus MMLV is a mixture of several temperature-resistant reverse transcriptase enzymes with good temperature adaptability. The optimal temperature range is 50-60 ℃, adapting to a variety of different reaction conditions. It has excellent reverse transcription performance.
Source
Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.
Concentration
200U/μl
Features
- Lack RNase H activity: Weak RNaseH activity High cDNA yield, can get more full length cDNA.
- Thermal stable: the optimum reaction temperature is 50℃,the highest is 60℃. Can overcome the template RNA secondary structure ,and finish the reverse transcriptase experiment smoothly.
- Wide temperature range: can reverse transcript from 37-60C,with more than 80% of the highest activity at 42℃-55℃ customer can choose the reaction temperature freely.
- Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA.Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.
Application
The first-strand cDNA synthesis; RT-PCR.
Unit definition
One unit of MMLV RT catalyzes the incorporation of 1 nmol of dTTP into acidinsoluble material in 10 minutes at 37℃ using oligo(dT)12-18-primed poly(A)n as a template.
Storage buffer
20 mM Tris-HCl (pH7.5),200 mM NaCl, 0.25 mM EDTA,0.01% NP-40(v/v),2.5 mM DTT,50% glycerol (v/v).
5×RT Buffer:250mM Tris-HCl (pH 8.3), 15mM MgCl2,375 mM KCl,50mM DTT.
