Protease Inhibitor Cocktail (100×)
2024-10-24
Single Cell WGA Kit(MDA)
2024-10-24Product Number: PCK07
Shipping and Storage
-20°C in dark; If used frequently, store at 2-8°C, avoiding repeated freezing and thawing.
Components
| Component | PCK07 100rxns | PCK07L 100rxns | PCK07H 100rxns |
| 2× One Step Buffer | 1.4 ml | 1.4 ml | 1.4 ml |
| One Step EnzymeMix | 100 μl | 100 μl | 100 μl |
| 50×Low ROX | - | 50 μl | - |
| 50×High ROX | - | - | 50 μl |
| RNase-Free Water | 1.5 ml | 1.5 ml | 1.5 ml |
Description
This product is a specialized reagent kit for one-step Real Time RT qPCR using probe methods (TaqMan, Molecular Beacon, etc.).When using this product for Real Time RT-qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cap during the reaction process, which avoids pollution and improves experimental efficiency.This product has high detection sensitivity, strong fluorescence signal, and high signal-to-noise ratio, making it very suitable for the detection of trace amounts of RNA such as RNA viruses. The special buffer system contained in it can maximize the effectiveness of both reverse transcriptase and DNA polymerase, improving reaction efficiency.Using this product can obtain a wider linear range, more accurate quantification of the target gene, good repeatability, and high reliability.
ROX dye is used to correct the fluorescence signal error generated between wells in quantitative PCR instruments, and is generally used in Real Time PCR amplification instruments from companies such as ABI and Stratagene.The excitation optical systems of different instruments vary, so the concentration of ROX dye must be matched with the corresponding fluorescence quantitative PCR instrument.
Instruments without ROX correction:Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, CFX96, etc.
Instruments requiring Low ROX correction:ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000, etc.
Instruments requiring High ROX calibration:ABI Prism7000/7300/770/7900, Eppendorf, ABI Step One/Step One Plus, etc.
Notes
- Please mix all the reagents in this kit gently upside down before use, trying to avoid foaming; then centrifuge briefly.
- This product uses RNA as a template for one-step RT-PCR experiments and should avoid RNase contamination during the operation. It is recommended to perform RNA operations in a dedicated area, using specialized instruments and consumables. operators should wear masks and one-time use gloves and change gloves frequently. Related consumables are treated with 0.1% DEPC (diethylpyrocarbonate) at 37°C for 12 hours and are autoclaved for 30 minutes.
- The reagents in this kit should avoid repeated freezing and thawing. Repeated freezing and thawing may degrade product performance.
- The kit must use specific primers. Primers can be selected according to specific experiments. Primers design will directly affect the result of RT-PCR reactions. When designing primers, many factors need to be considered, such as GC content, primer length, primer position, secondary structure of PCR products, etc. It is recommended to use professional software to design.
- This kit recommends the use of specific probes. It is recommended to use professional software for design.
