Manual

Product Number: PCK53

Storage condition

Store at -20°C.

Component

Description

The MicroRNA cDNA Kit for First Chain cDNA Synthesis is a specialized kit based on the principle of the stem loop method. The 5 × THERMO Reaction Mix is a tubular reverse transcription premix, containing all the reagents required for reverse transcription (THERMScript H-RTase, RNase inhibitor, dNTP Mixture, Buffer). Simply add template RNA and stem loop primers for the reaction, making cDNA synthesis more convenient and efficient. This kit uses a special gDNA Remover with DNA degradation activity, which does not require opening the lid halfway to add. With just one step, genome clearance and reverse transcription reactions can be completed simultaneously, greatly simplifying the operation steps and avoiding the risks of sample contamination and RNA degradation caused by complex sample addition processes. This product is based on THERMOscript H ˉ RTase, which has extremely high thermal stability. Coupled with an optimized buffer system, it maximizes the synthesis of various microRNA specific reverse transcription products. The cDNA product has good compatibility and can be paired with any commercial company's fluorescence quantitative mix for downstream detection experiments.