Manual

Product Number: PCK50

Storage condition

-20°C

Component

Description

         Enhanced microRNA 1st Strand cDNA synthesis kit This kit uses the Poly (A) tail addition principle. Firstly, a Poly (A) tail is added to the 3 'end of microRNA, and then an Anchored oligo (dT) - universal tag universal reverse transcription primer is used for reverse transcription reaction to generate the corresponding cDNA first chain of microRNA. This kit uses a specially optimized pre mixed microRNA RT Enzyme Mix to combine Poly (A) tailing and reverse transcription into one step, simplifying the operation steps and improving the efficiency of Poly (A) tailing and reverse transcription. The kit has the ability to effectively prepare microRNA corresponding cDNA first strands from 20pg-2μg Total RNA. A single synthesized cDNA can detect multiple microRNAs, saving samples and costs.

Note: This kit must be used in conjunction with the Enhanced microRNA Real-Time PCR Assay kit(PCK51).