Manual

Product Number:RT05F

Shipping and Storage

Stored at -30~-15℃, Dry ice transportation to avoid repeated freezing and thawing.

Components

Description

HiFi II M-MLV Reverse Transcriptase (RNase H-) (Glycerol-free) is the glycerol free version of HiFi II M-MLV Reverse Transcriptase (RNase H-),it is a reverse transcriptase that recombines and expresses the mutated M-MLV gene using Escherichia coli engineering bacteria,this enzyme can catalyze complementary DNA polymerization reactions using RNA or DNA: RNA hybrid chains as templates.The mutated HiFi II M-MLV (H -) reverse transcriptase RNase H activity is missing, reducing RNA degradation in reverse transcription reactions and making it easier to obtain full-length cDNA.HiFi II M-MLV (H -) reverse transcriptase can synthesize the first strand of cDNA at 55℃, providing higher specificity and stability. It can synthesize up to 12kb of cDNA with high cDNA yield.Suitable for the synthesis of first strand cDNA, RT-PCR, RT-qPCR, construction of full-length cDNA libraries, and application of freeze-dried RT-PCR products.This product does not contain freeze-dried shaping ingredients, and can be customized and added according to needs when applied to freeze-dried products.

Unit definition

Using Poly (A) as the template and oligo (dT) as the primer, the enzyme required to catalyze the incorporation of 1nmol of dTTP within 10 minutes at 37 ℃ is defined as one active unit (U).

Quality Control

The electrophoresis bands of the RNA did not change when 200U of this enzyme reacted with 1µg of 16S, 23S rRNA at 37 ℃ for 1 hour.

Notes

1. During the operation process, RNase contamination should be avoided to prevent RNA degradation or cross contamination during experiments. It is recommended to perform RNA operations in specialized areas using specialized instruments and consumables. Operators should wear masks and disposable gloves and frequently change gloves.
2. Disposable plastic containers should be used as much as possible for experiments. If glass containers are used, they should be treated with a 0.1% DEPC (diethyl pyrocarbonate) aqueous solution at 37℃ for 12 hours and sterilized under high pressure at 120℃ for 30 minutes before use, or dry heat sterilized at 180℃ for 60 minutes before use. The sterile water used in the experiment should be treated with 0.1% DEPC and subjected to high-pressure sterilization.
3. Before use, all reagents in this reagent kit should be gently mixed upside down to avoid foaming and used after brief centrifugation. The enzymes involved should be returned to -20℃ as soon as possible after use to avoid repeated freezing and thawing.
4. If the initial amount of RNA is less than 50ng, it is recommended to add RNA enzyme inhibitors (RNase inhibitor, Murine).