Manual

Product Number:PCK27

Shipping and Storage

-30°C~ -15°C, transport ≤0°C.

Component

Description

Fast Probe One Stept RT-qPCR, UNG Kit is designed specifically for quantitative PCR detection using RNA as a template, such as RNA viruses. Using gene-specific primers (GSPS), reverse transcription and qPCR reactions are performed in one tube without the need for additional tube opening/pipetting operations, greatly increasing assay throughput and reducing the risk of contamination. The dUTP/UNG anti-contamination system is introduced in this kit. Heat sensitive UNG can degrade U-containing pollutants rapidly at room temperature. Rapid inactivation of UNG by reverse transcription at 55°C did not affect the efficiency and sensitivity of qRT-PCR. Combined with optimized buffer system, antibody modified Taq enzyme and mutated M-MLV, the detection sensitivity of Fast Probe One Stept RT-qPCR, UNG Kit can reach 0.1pg total RNA or <10 copies of RNA template and has higher thermal stability. 5×Fast Probe One Stept RT-qPCR UNG Buffer contains optimized buffer system and dNTP/dUTP Mix, which is especially suitable for high specificity, low template concentration and multiple fast detection of fluorescent-labeled probes such as TaqMan.

Note

Before use, please gently mix the product upside down after it is completely melted, avoid foaming as far as possible, and use after a short centrifugation. Avoid freezing and thawing this product repeatedly. ROX dye is used to correct fluorescence signal errors generated between quantitative PCR holes. This product does not contain ROX dye.