Manual

Product Number:PCK54

Shipping and Storage

-30°C~ -15°C.Avoid repeated freeze-thaw cycles.

Component

Description

This product is a kit for one-step Real Time RT qPCR using RNA as a template using probe methods such as TaqMan and Molecular Beacon. When using this product for Real Time RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, without the need to add reagents or open the tube cap, which avoids contamination and improves experimental efficiency. This reagent introduces a dUTP/UG anti contamination system, which can rapidly degrade pollutants containing U at room temperature without affecting the efficiency and sensitivity of RT qPCR. This product is equipped with a freeze-drying protectant and can be used for the preparation of freeze-drying reagents.

Note

1. This product uses RNA as a template for one-step RT-PCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a dedicated area, using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with a 0.1% DEPC (diethyl carbonate) aqueous solution at 37 ℃ for 12 hours, and sterilized under high pressure for 30 minutes before use.
2. This product should avoid repeated freeze-thaw cycles and is recommended to be packaged and stored separately.
3. If there is precipitation or crystallization after melting the 5 × One Step Lyophilization Protector, please dissolve it in a 70 ℃ water bath without affecting its use.