Manual

Product Number: PCM80

Shipping and Storage

-20 ± 5℃. If frequent use is required, it can be stored at 2-8℃ to avoid repeated freezing and thawing.

Component

Description

Universal FastStart Probe Mixture (UNG) is a 2 × qPCR premix suitable for fluorescence quantitative detection using probe method, including Taq DNA Polymerase、Uracil-N-Glycosylase, PCR Buffer, dNTPs, Mg^2+, K^+, Enhancers and stabilizers, etc. Taq DNA Polymerase is a double antibody blocking type hot start enzyme, with a polymerase activity blocking rate of over 95% at temperatures of 55℃ and below. It can effectively reduce non-specific amplification at low temperatures. The added Uracil-N-Glycosylase and dUTP anti fouling system can catalyze dsDNA and ssDNA containing uracil, release free uracil, and reduce cross contamination of amplification products. The unique PCR buffer system can significantly improve the efficiency of qPCR amplification and accurately detect within a dynamic range of up to 6 logarithmic levels.

This product is suitable for single and multiple amplification, with high detection sensitivity and good specificity. It can detect templates as low as single copy and supports direct amplification of oral swabs and low concentration blood samples. It has strong universality and is widely used in gene expression and virus detection. And the glycerol content of the premix is extremely low, which can be directly combined with freeze-drying protectants for freeze-drying.