Manual

Product Number: PCM42

Shipping and Storage

-20℃。

Components

Component	PCM42
2×PCR Plus Master Mix,Dye	5×1mL
ddH2O	5×1mL

Description

This product is a premixed system composed of efficient PCR Plus Master Mix, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2×. PCR Plus Master Mix is a hot start enzyme modified by antibodies that can perform high specificity Hot Start PCR. This enzyme exhibits both 5 '-3' polymerase activity and 3 '-5' exonuclease activity, with a fidelity approximately 52 times that of Taq DNA Polymerase and an amplification length of up to 20 kb.

In addition, this product uses a high stress resistant buffer and exhibits excellent performance in the amplification of crude samples, which can effectively amplify crude samples containing multiple PCR inhibitors that are difficult to amplify with ordinary PCR enzymes.

This product contains electrophoresis indicator, and agarose gel electrophoresis can be carried out directly after PCR reaction.

Features

1. High stress resistance

The amplification ability of crude extracted animal and plant tissues is very strong. For samples such as blood and microorganisms, there is no need to extract nucleic acid, and sufficient amplification products can be obtained by directly adding them to the reaction solution.

• High amplification ability

For short fragments and purified templates, the extension speed can reach 10-15 seconds/kb; For templates with longer or more complex fragments, it is recommended to use an extension speed of 30 seconds/kb; At the same time, it can also effectively amplify trace templates.

• High specificity

Efficient hot start enzymes significantly improve amplification specificity.

• High-fidelity

The fidelity of this product is approximately 52 times that of Taq DNA Polymerase. Applicable to experiments where PCR products are cloned on a vector and then sequenced.

• High stability

Place this product at 37℃ for accelerated stability testing for 10 days without affecting its performance.