Manual

Product Number: PCM34

Storage

-20°C, try to avoid repeated freezing and thawing.

Component

Description

2×Multiplex PCR MasterMix (UNG) is a PCR premix system composed of GoldStar Taq DNA Polymerase, Mg2+, dNTPs (including dUTP), UNG enzyme and PCR stabilizer. The use of this product does not require the optimization of complicated PCR reaction conditions, and multiple PCR reactions can be easily performed by simply exploring the conditions.

The GoldStar Taq DNA Polymerase contained in this product is a chemically modified hot-start enzyme, which can effectively reduce the non-specific amplification caused by primer mismatch in the early stage of PCR reaction. The unique buffer system enables all primers in the multiplex PCR reaction to be effectively extended without additional optimization. Also included in this MasterMix is the GC Enhancer, which facilitates efficient amplification of "difficult" templates (eg, those with high GC content). This product uses the dUTP-UNG anti-pollution system, which can effectively remove the residual pollution of PCR products and greatly reduce the false positives caused by the pollution of amplification products. The UNG enzyme can be inactivated by the pre-denaturation step in the PCR cycle, so it will not affect the formation of new dU-containing PCR products.

Multiplex PCR MasterMix (UNG) can effectively prevent residual contamination of PCR products, and is suitable for anti-pollution multiplex PCR reactions, such as microsatellite analysis, genotyping and SNP detection, etc.

Quality Control

After testing, there is no exogenous nuclease activity; PCR method detects no host residual DNA; storage at 2-8°C for 3 days, no significant change in amplification performance.