Manual

Product Number: PCM08

Shipping and Storage

-20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing.

Components

Description

The Master SYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains Fast Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg²⁺. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The Fast Taq DNA Polymerase in the mixture that does not have polymerase activity at room temperature which prevents non-specific amplification efficiently, and it is activated by incubation at 95°C only for 30 seconds which greatly reduces the reaction time. The combination of a unique PCR buffer system and a hot-start enzyme effectively inhibits non-specific PCR amplification and significantly increases the amplification efficiency of PCR.

Notes

1. Mix gently before use, avoid foaming, and use after brief centrifugation.
2. This product contains SYBR Green I fluorescent dye. Avoid strong light irradiation when storing this product or preparing PCR reaction solution.
3. Avoid frequently freezing and thawing of this product. It may comprise product performance.
4. This product cannot be used for probe based qPCR .
5. When preparing the reaction solution, use new or non-contaminated tips and centrifuge tubes to prevent contamination.

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