Hotstar DNA Polymerase
2024-10-25
NGS Library Quantitative Kit for Illumina
2024-10-25
Product Number: PCK83
Shipping and Storage
-20℃, 12 months. If frequently used, it can be stored at 2-8 ℃ to avoid repeated freeze-thaw as much as possible.
Components
| Component | PCK83 | PCK83 |
| 2×GoldStar TaqMan Mixture | 1ml | 5×1 ml |
| Primer Mix 1 | 300µl | 5×300 µl |
| Human DNA Standard 1(100ng/µl) | 100µl | 5×100 µl |
| 50×High rox | 40µl | 200 µl |
Description
This product uses the probe method for real-time fluorescence quantitative PCR (qPCR) to accurately detect the concentration and quality of DNA extracted from various samples (paraffin samples, flow cytometry sorted cells, serum or plasma samples, and small amounts of clinical samples, etc.). The product provides a complete set of reagents required for the qPCR process, including reaction mixture, primer mixture, and standard samples. Simply add the extracted DNA to start the experiment, which is simple, convenient, time-saving, and labor-saving. The reaction mixture uses an efficient and fast hot start Golden Star Taq DNA Polymerase, which has high amplification sensitivity and specificity, and shortens the time of the programmed reaction.
ROX dye is used to correct the fluorescence signal error generated between wells in quantitative PCR instruments, and is generally used in Real Time PCR amplification instruments from companies such as ABI and Stratagene. The excitation optical systems of different instruments vary, so the concentration of ROX dye must be matched with the corresponding fluorescence quantitative PCR instrument.
Instruments that do not require ROX calibration:Roche LightCycler 480，Roche LightCyler 96，Bio-rad iCyler iQ，iQ5，CFX96 etc.
Instruments that require Low ROX calibration:ABI Prism7500/7500 Fast，QuantStudio® 3 System，QuantStudio®5 System，QuantStudio®6 Flex System，QuantStudio®7 Flex System，ViiA 7 system，Stratagene Mx3000/Mx3005P，Corbett Rotor Gene 3000 etc.
Instruments that require High ROX calibration:ABI Prism 7000/7300/7700/7900，Eppendorf，ABI StepOne/Step One Plus etc.
Note:The preparation methods for High Rox and Low Rox are described in Operation Step 3.
Application
This product is suitable for quantitative detection of human genomic DNA sample concentration in fields such as scientific research, clinical practice, forensic science, and parent-child identification.
