Description:

The kit already include MMLV, HotStar DNA polymerase, RNAse inhibitor, dNTPs, MgCL and so on. Which special optimized for COVID-19 Virus. Customer only need add oligos, probe, and positive control and negative control then can be your own detection kit. This kit offered by bulk pack, easy for your make the detection kit. If need oligo, probe, template we also can offer.

Hot Star Probe One Step RT-qPCR Kit is designed for one-step qPCR based on probes (TaqMan, Molecular Beacon etc.). The reverse transcription and quantitative PCR are carried out in the one reaction system, no need to add other reagents in the whole reaction process. The unique reverse transcriptase in the kit greatly improved transcriptional activity. The new hot start polymerase has the advantages of high amplification efficiency and good specificity. The special buffer system contained in the kit can make the reverse transcriptase and DNA polymerase play the maximum role at the same time and improve the reaction efficiency. GoldStar Probe One Step RT-qPCR Kit is highly sensitive, strong fluorescent signal and highly signal-to-noise ratio. It is very suitable for detecting RNA such as RNA virus, new coronavirus (20I9-nCoV). Using this product, we can get a wider linear dynamic range, more accurate quantitative target gene, good repeatability and high reliability.

Feature:

• (1) One Step RT-PCR allows accurate, rapid analysis of small amounts of RNA such as viral RNA.
• (2) Hotstar Polymerase enables highly efficient, specific amplification and detection.
• (3) One Step RT-PCR Buffer is a 2X premix, which allows simple preparation of the reaction with minimal risk of contamination.
• (4) easy to use, the kit already include MMLV, HotStar DNA polymerase, RNAse inhibitor, dNTPs, MgCL and so on. Special optimized for COVID-19. Customer only need add oligos, probe, and positive control and negative control then can be your own detection kit. If need oligo,probe, template we also can offer.

Quality Control:

Function test by RT-qPCR use WHO and CDC Recommended template, and primer