Manual

Product Number: PCM43R

Shipping and Storage

Store at -20°C for 2 years. For short-term use (within 1 month), it can be kept at 4°C.

Component

Description

This product contains HiFi Long DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2 ×. When using, simply add the template and primer, and add water until the final concentration of Mix is 1 ×. This product is a mixture of various ultra fidelity enzymes modified with advanced genetic engineering and extended factors, greatly improving amplification length, amplification speed, fidelity, and yield. This product is the preferred product for long fragment ultra fidelity rapid amplification. This product contains a red tracer dye and can be directly loaded for electrophoresis without the need for sample buffer; It can also be purified for subsequent operations such as enzyme digestion, ligation, fluorescence sequencing, etc. The PCR product is a flat end and does not require the addition of an A head. It can be directly cloned using the pTOPO Blunt flat end series vector.

Features

1. Fast amplification speed: The extension speed can reach 3-4kb/min, which is 6-8 times faster than PFU.
2. High amplification yield: Generally, the PCR product yield is 50% -100% higher than that of traditional pfu.
3. Excellent authenticity: The fidelity is over 54 times that of taq. Generally, randomly selecting a bacterium for sequencing is the correct and mutation free colony.
4. Long amplification length: Complex genomic DNA is suitable for amplifying products up to about 10kb, while simple genomic, plasmid, and phage DNA are suitable for amplifying products up to about 15kb.