Manual

Product Number: PCM20

Shipping and Storage

-20°C.

Components

Description

This product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample processed by gDNA remover can be directly used for reverse transcription reaction to synthesize cDNA.

This kit contains a novel high-performance reverse transcriptase HiFiScript. 5×HifiScript RT MasterMix contains all the components needed for reverse transcription. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. If the cDNA is used for downstream qPCR, the reverse transcription reaction can be completed in 15 minutes. This kit is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.

Features

1. Rapid genomic DNA deletion: With the gDNA remover, it takes only 2 minutes to remove genomic DNA.
2. Rapid reverse transcription: It takes only 15 minutes to obtain the first strand of cDNA.
3. Easy to use: RT MasterMix contains all the components needed for reverse transcription and ready to use.
4. High sensitivity: pg of total RNA or mRNA can be used as template.
5. High efficiency of reverse transcription efficiency: the novel mutation site enhancers the activity of the enzyme, to increase the yield of cDNA.

Note

1. RNase contamination should be avoided during operation to prevent RNA degradation or cross-contamination in experiments. We suggest that the RNA experiments should be performed in a specialized area with specialized equipment and consumables. The operator should wear a mask and disposable gloves and change gloves frequently.
2. Try to use disposable plastic containers. If glassware is used, it should be treated at 37°C for 12 hours with 0.1% DEPC and autoclaved at 120°C for 30 minutes before use, or glassware is dry heat sterilized at 180°C for 60 minutes before use. Sterile water used in the experiment should be treated with 0.1% DEPC, then be autoclaved.
3. The reaction should be set up on ice to prevent RNA degradation. The enzymes should be returned to -20°C as soon as possible after use to avoid repeated freezing and thawing.
4. For RNA templates with complex secondary structures, it is recommended to incubate the template RNA for 5 minutes at 65°C first, then place it on ice immediately, and centrifuge briefly for further processing.