Manual

Product Number: PCM55

Shipping and Storage

-20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing.

Components

Description

The Fast SYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains Fast Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg²⁺. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The Fast Taq DNA Polymerase in the mixture is a chemically-modified, new efficient enzyme which reduce non-specific amplification efficiently, and it is activated by incubation at 95°C for 20 seconds, which will efficiently reduce the total reaction time.

This product is suitable for fluorescent qPCR instruments with no ROX.

No ROX as a calibration dye: Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, and CFX96.

Need Low ROX (30-50mM) as a calibration dye: ABI Prism7500/7500 Fast, QuantStudio® 3System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System,ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000

Need High ROX (300-500mM) as a calibration dye: ABI Prism7000/7300/7700/7900，Eppendorf，ABI Step One/Step One Plus.

Features

This product uses a new high-performance hot start enzyme (Golden Star Taq DNA Polymerase) and a unique PCR buffer system. This product significantly improves the PCR amplification efficiency and has high sensitivity and specificity. This product is suitable for quantitative PCR detection and can accurately quantify and detect the target gene.

Note

1. Mix gently before use, avoid foaming, and use after brief centrifugation.
2. This product contains SYBR Green I fluorescent dye. Avoid strong light irradiation when storing this product or preparing PCR reaction solution.
3. Avoid repeated freezing and thawing of this product. Repeated freezing and thawing may comprise product performance.
4. This product cannot be used for qPCR using probes.
5. When preparing the reaction solution, use new or non-contaminated tips and centrifuge tubes to prevent contamination.