Manual

Product Number: PCM99

Shipping and Storage

-20℃, if frequently used, can be stored at 2-8℃ to avoid repeated freeze-thaw as much as possible.

Components

Description

The Fast Probe Mixture is a premixed system specifically designed for real-time fluorescence quantitative PCR using probe methods (TaqMan, Molecular Beacon, etc.), with a concentration of 2×,Contains specific Taq DNA Polymerase, PCR Buffer, dNTPs, Mg²⁺, as well as enhancers and stabilizers, making the operation simple and convenient.Mainly used for detecting genomic DNA target sequences and cDNA target sequences after RNA reverse transcription. The specific Taq DNA Polymerase contained in this product can effectively reduce non-specific amplification caused by non-specific binding of primers and templates or primer dimers under room temperature conditions. The activation of the enzyme only needs to be incubated at 95℃ for 30 seconds, greatly shortening the reaction time of PCR.The unique combination of PCR buffer system and hot start enzyme effectively inhibits the production of non-specific products, significantly improving the amplification efficiency of PCR, with stronger fluorescence signal, higher sensitivity, and wider linear range.

Notes

1. Before use, please gently mix it upside down and avoid foaming as much as possible. After briefly centrifuging, use it.
2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored in a dark place at -20℃ for long-term storage. If frequent use is required in the short term, it can be stored at 2-8℃.
3. ROX dye is used to correct the fluorescence signal error generated between wells in the quantitative PCR instrument, and this product does not contain ROX dye.