2×PCR Super MasterMix, with blue dye
2024-10-29
Fast NGS Library Kit for Illumina&MGI
2024-10-30Product Number:PCK13
Shipping and Storage
Box 1：Store at -20°C; transportation conditions: dried ice.
Box 2：Store at 2°C~8°C; transportation conditions: ice bag.
Components
Box 1: Cyclization reagents
| Component | PCK13 |
| Splint Oligo | 20μL |
| T4 DNA Ligase | 250μL |
| 5×Splint Buffer | 50μL |
| Digestion Buffer | 20μL |
| Digestion Enzyme Ⅰ | 70μL |
| Digestion Enzyme Ⅲ | 25μL |
Box 2: DNA Purification Beads
| Component | PCK13 |
| Magnetic Bead | 1.5mL/tube ×4 |
Description
Cyclation kit is a modular kit tailored for MGI's high-throughput sequencing platform. This kit can be used to prepare PCR products from the spliced joint into single-stranded circular DNA libraries suitable for MGI sequencers. All reagents provided in the kit have undergone strict quality control and functional verification to ensure maximum stability and repeatability of library construction.
Consumables and Equipment
- Magnetic rack: DynaMagTM-2 is recommended.
- Qubit®3.0 ThermoFisher.
- Qubit®3.0 ssDNA Assay Kit.
- Anhydrous ethanol, EB (10mM Tris-HCl, pH 8.0), NF Water (pH 7.0 to 8.0) .
- Reaction tube: It is recommended to use low adsorption PCR tube and EP tube.
Tips: It is recommended to use high quality filter gun head placement kit, library sample contamination
Preparation
- Sample preparation
- PCR products: a total of 330 or more ng (multiple PCR product mix, refers to the total) after mixing, the volume of 49μL (if the PCR product itself insufficient volume, supplementary NF Water to total volume 49μL).
- PCR products fragment size: fragment peaks between 200-500bp.
- Modification: PCR products has joined for MGISEQ-2000, and BGISEQ MGISEQ -200-500 such as sequencing platform (Index) of fixed sequence.
- Preparation of reagents
- Take out corresponding reagent kit, centrifugation, enzyme mixture on the ice to use: Before use, the buffer should be dissolved at room temperature, centrifuged by shock, placed on ice for use, NF Water and EB at room temperature for use.
- Please compound mixture on the ice.
- Please make reaction system on the ice.
- Kit buffer after dissolving in possible precipitation, precipitation does not affect the reagent function, please fully vibration blending to precipitation disappear after use.
Workflow
