SNP Genotyping qPCR MIX, UNG
2024-11-04
Fast Probe One Stept RT-qPCR, UNG Kit
2024-11-04Product Number: BSM03
Shipping and Storage
-20°C.
Components
| Component | BSM03 200μL |
| Bst 3.0 Enzyme Mix | 200μL |
| 10×Bst 3.0 Reaction Buffer | 1.5mL |
| 100mM MgSO4Solution | 1.5mL |
Description
Bst 3.0 Enzyme Mix is a mixed enzyme containing Bst DNA polymerase and high-temperature resistant reverse transcriptase. Bst DNA polymerase is a recombinant enzyme expressed and purified through E. coli, which undergoes partial point mutations on the basis of the original sequence. It has stronger 5'→3' DNA polymerase activity, strand displacement activity, reverse transcriptase activity, and no 5'→3' exonuclease activity.High temperature resistant reverse transcriptase is a new enzyme modified by genetic engineering, with fast cDNA synthesis speed and significantly improved thermal stability. It can withstand reaction temperatures up to 60℃ and is suitable for reverse transcription reactions of RNA templates with complex secondary structures.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.Bst 3.0 Enzyme Mix can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.
Unit definition
This product is suitable for various isothermal amplification reactions such as RT LAMP, LAMP, RCA, CPA, etc.
Heat Inactivation
Incubate at 80℃ for 5 minutes before inactivation.
