Manual

Product Number:PCM22

Shipping and Storage

-20±5˚C. Short term use can be stored in 2-8˚C. Try to avoid repeated freeze-thaw cycles as much as possible.

Components

Description

The 2×Animal Detection Probe Mixture, UNG (Mg²⁺ free) Lyophilized is a specialized freezeable intervention mixture that does not contain Mg²⁺ and is suitable for probe detection of DNA viruses, with a concentration of 2×,contains novel antibody modified Taq DNA polymerase, PCR buffer, dNTPs, as well as enhancers and stabilizers, making it convenient and fast to use. This product does not contain Mg²⁺, and the Mg²⁺content can be adjusted to meet different amplification needs.This product is compatible with single and multiple probe qPCR reaction systems, and can also be used for the preparation of freeze-drying detection reagents.

This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.

Preparation and precautions before use

1. Before use, please gently mix the product upside down after it has completely melted, avoiding foaming as much as possible, and use it after a brief centrifugation.
2. AD freeze-drying protective agent can be melted in a 70˚C water bath for later use.
3. This product can be stored for a long time at -20˚C. If frequent use is required in the short term, it can be used between 2-8˚C save.
4. ROX dye is used to correct the fluorescence signal error generated between quantitative PCR wells, and this product does not contain ROX dye