Manual

Product Number:PCM21

Shipping and Storage

Store at 4-30℃, and store at -20℃ until fully used after re dissolution, avoiding repeated freeze-thaw as much as possible..

Components

Description

Animal Detection Probe Mixture (UNG), Lyophilized is a specialized in situ full component freeze-drying reagent suitable for detecting DNA viruses using probe method. It includes novel antibody modified Taq DNA polymerase, PCR Buffer, dNTPs, Mg²⁺, as well as enhancers and stabilizers.Convenient and fast to use, simply add primer probes and extract the nucleic acid samples for machine amplification. This product is compatible with single and multiple probe qPCR reaction systems.

This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction.This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.

Note

1. This product can be stored at 4-30℃ for a long time, and at -20℃ for longer periods of storage. If it cannot be used up after re dissolution, it can be stored at -20℃ to avoid repeated freeze-thaw.
2. ROX dye is used to correct the fluorescence signal error generated between quantitative PCR wells, and this product does not contain ROX dye.