Manual

Product Number:PCM58

Shipping and Storage

-20°C, if frequently used, can be stored at 2-8℃ to avoid repeated freeze-thaw as much as possible.

Components

Description

Animal Detection Probe Mixture (UNG) is a specialized premix suitable for probe detection of African swine fever virus (ASFV), with a concentration of 2×,Contains novel antibody modified Taq DNA polymerase, PCR Buffer, dNTPs, Mg²⁺, as well as enhancers and stabilizers, making it convenient and fast to use.This product can be compatible with single and multiple probe qPCR reaction systems, and can also be used for detecting genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

This product uses the dUTP-UNG anti pollution system, and dUTP is added during the preparation process of the PCR reaction system, resulting in the formation of amplification products containing dU bases.And this product can be eliminated by UNG enzyme treatment in the PCR system before the next PCR reaction. This effectively removes residual contamination of PCR products and greatly reduces false positives caused by amplification product contamination.The pre denaturation step of UNG enzyme in the PCR cycle can be inactivated, so it will not affect the formation of new dU based PCR products.

This product contains highly sensitive engineered DNA polymerase modified with antibodies, which can effectively reduce non-specific amplification generated by non-specific binding of primers and templates or primer dimers under room temperature conditions, while significantly improving detection sensitivity and amplification efficiency. The activation of the enzyme only needs to be incubated at 95℃ for 30 seconds, greatly shortening the reaction time of PCR.The carefully optimized PCR buffer system effectively inhibits the production of non-specific products, significantly improving the amplification efficiency and sensitivity of PCR.

Notes

Before use, please gently mix the product upside down after it has completely melted, avoiding foaming as much as possible, and use after briefly centrifuging.Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored in a dark place at -20℃ for long-term storage.If frequent use is required in the short term, it can be stored at 2-8℃. ROX dye is used to correct the fluorescence signal error generated between quantitative PCR wells, and this product does not contain ROX dye.