Manual

Product Number: PC58             for qPCR First Strand cDNA Synthesis Kit

Storage condition

Store at -20℃, valid for 12 months.

Component

Component	PC5801 50rxn	PC5802 100rxn
5×RT MasterMix	200μl	400μl
RNase free H2O	1 ml	2×1 ml

Description

The 5×RT MasterMix offer a whole component for cDNA synthesis. Which is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The Mix utilizes the recombinant thermostable M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase.

This MasterMix already include all the reaction component. the thermostable M-MuLV Reverse Transcriptase, RNase Inhibitor, Random primers, Oligo dT Primer, dNTP Mixture, Buffer. When set up the reaction only need add water and RNA template.

Feature

1.      Themo stable MMLV, high efficiency and stable.

2.      Whole component Mix, simple for reaction setup, fast reaction.

3.      High concentration, much more volume for RNA template, suitable for low copy RNA template amplification.

4.      The premix is not frozen under-20℃, simplified the Thaw and mixing progress.

5.      Specially optimize for qPCR

Application

First Strand cDNA Synthesis. Detecting high copy and low copy genes.

RT-qPCR

Take a suitable amount of cDNA products (generally not more than 1/10 of the volume of qPCR reaction) as the template of qPCR. If the expressed genes are abundant, they can be diluted according to actual conditions.

Note

1. Avoid of RNase pollution
2. To make sure reaction successful, the high-quality RNA template is suggested.
3. 5×RT MasterMix is Very viscous, and easily adsorbed on the tube and pipe tips causing loss. Pleas centrifuge shortly before use. The enzyme in the 5×RT MasterMix are all excessive 3.6μl-3.8μl per prep will not affect the reaction.