Manual

Product Number: PCM88

Shipping and Storage

Stored at -20℃, transported in ice bags, Mix can be stably stored for 6 months under dark conditions at 2~8℃ after thawing.

Components

Note: contains dNTP、Mg²⁺、FastStar DNA Polymerase、ROX Reference Dye etc.

Description

The 2× Universal Fast SYBR Master Mix is a specialized premix for real-time fluorescence quantitative qPCR reactions using the dye method (SYBR Green I).The core component FastStar DNA Polymerase is an antibody based hot start DNA polymerase that can be restored by heating at 95℃ for 5 seconds. It has many advantages such as strong specificity and high detection sensitivity, and is paired with an optimal buffer optimized for qPCR. The reaction solution concentration is 2×。The unique qPCR buffer system of this product, combined with hot start enzymes, effectively inhibits the production of non-specific products and significantly   improves the amplification efficiency of qPCR. It is very suitable for high specificity and sensitivity qPCR reactions.

This product is also suitable for qPCR rapid reaction program, 10 μ L small volume reaction can achieve rapid and accurate detection and quantification of target genes without affecting specificity and high sensitivity. Good standard curves can be obtained within a wide quantitative range, with accurate, reproducible, and reliable quantification of target genes.

Application

This reagent is used for quantitative amplification of DNA samples and can amplify DNA from most species. Sample types can be genomic DNA, cDNA, plasmid DNA λ DNA, etc.

Note

1. Before use, please gently mix it upside down and avoid foaming as much as possible. After briefly centrifuging, use it. During the sample addition process, blow gently. If Mix bubbles due to improper operation, it needs to be centrifuged again before use.
2. Try to avoid repeated freeze-thaw cycles of this product, with freeze-thaw cycles (≤ 10) to avoid a decrease in enzyme activity. It is recommended to use small portions separately.
3. Due to the presence of fluorescent dye SYBR Green I in this product, it is necessary to store it away from light. When preparing the reaction system, it is advisable to avoid strong light exposure as much as possible.
4. Due to its extremely high detection sensitivity, this product is easily contaminated by aerosols in the air. Therefore, when preparing the reaction system, please use a super clean workbench. During the preparation process, please use a sterilization gun head and reaction tube. If conditions permit, it is recommended to use a dedicated pipette gun and a gun head with a filter element in laboratories.
5. This product contains ROX Reference Dye that is suitable for most models.