Manual

Product Number: PCM56

Shipping and Storage

-20°C; For frequent uses, store at 2-8°C.

Component

Note: 2×HotStar Best MasterMix contains HotStar Best DNA Polymerase, 3.4 mM MgCl₂and 400μM each dNTP.

Description

This product is a premixed system composed of HotStar Best MasterMix, Mg²⁺, dNTPs, and PCR stabilizers and enhancers at a concentration of 2×. This product has the advantages of simple and quick operation, high sensitivity, high specificity and good stability, which decrease human error and contamination maximumly. The HotStar Best DNA Polymerase has 5’-3’ DNA polymerase activity, 5’-3’ exonuclease activity and 3’-5’ exonuclease activity. Compared with HotStar Best MasterMix, this polymerase has higher amplification efficiency and lower mismatch rate under normal PCR conditions. The chemical-modified enzyme does not have polymerase activity at room temperature, thus effectively avoiding non-specific amplification. The polymerase must be activated by incubation at 95°C for 10 minutes.

The optimized buffer system maximizes the effect of the enzyme and achieves high fidelity, high specificity, high amplification efficiency, and high sensitivity amplification of the target fragment. This product does not contain dyes, and an appropriate amount of

sample loading buffer should be added for electrophoresis. Most of the amplified PCR products have an "A" base attached to the 3' end, and therefore can be directly used for T/A cloning. It is mainly suitable conventional PCR reactions and experiments such as

gene cloning that require high fidelity.

Quality Control

No exogenous nuclease activity was detected; No host DNA was detected by PCR; single copy genes in multiple genomes could be efficiently amplified; No apparent activity change after being stored at 2-8°C for three months.