E Taq DNA Polymerase
2024-10-22
2x E Taq Master mix, without dye
2024-10-22Product Number: PCM12B
Shipping and Storage
-20°C
Components
| Component | PCM12B (1mL) | PCM12B(5mL) | PCM12B(25mL) |
| 2×E Taq Master mix, with blue dye | 1mL | 5×1mL | 5×5mL |
| ddH2O | 1mL | 5×1mL | 5×5mL |
Note:2×E Taq Master mix, with blue dye contains E Taq DNA Polymerase , 3mM MgCl2 and 400μM each dNTP.
Description
This product is a premixed system composed of E Taq DNA Polymerase, Mg2+, dNTPs, and PCR stabilizers and enhancers at a concentration of 2x. E Taq DNA Polymerase has high amplification efficiency and low mismatch rate. The original MasterMix formula makes the whole reaction system very stable. More than 98% of PCR amplifications can be successful at one time. At the same time, complicated templates can be effectively amplified, and human error and contamination can be minimized. This product has been added with dye (blue), and electrophoresis gel detection can be performed directly after the reaction. Most of the amplified PCR products have an "A" base attached to the 3' end, and therefore can be directly used for T/A cloning. It is mainly suitable conventional PCR reactions and experiments such as gene cloning that require high fidelity.
Quality Control
No exogenous nuclease activity was detected; no host DNA was detected by PCR; single copy genes in multiple genomes could be efficiently amplified.
