Multiplex PCR Mix
2024-10-24
Hot Exo- DNA Polymerase
2024-10-24Product Number: PCM33H
Shipping and Storage
-20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing.
Components
| Component | PCM33H 1ml | PCM33H 5ml | PCM33H 25ml |
| 2×Ultra Sybr qPCR Mix (Hgh Rox) | 1mL | 5×1mL | 5×(5×1ml) |
| ddH2O | 1mL | 5×1mL | 5×(5×1ml) |
Description
The 2×Ultra Sybr qPCR Mix (Hgh Rox) is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains Golden Star Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, Mg2+ and High ROX as reference dye. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.
This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The Golden Star Taq DNA Polymerase in the mixture is a chemically-modified,new efficient hot-start enzyme that does not have polymerase activity at room temperature which prevents non-specific amplification efficiently, and it is activated by incubation at 95°C for 10 minutes. The combination of a unique PCR buffer system and a hot-start enzyme effectively inhibits non-specific PCR amplification and significantly increases the amplification efficiency of PCR.
The ROX dye contained can correct the fluorescence signal error between the wells of the quantitative PCR instrument. The amount of ROX in this kit is low, and it is suitable for quantitative PCR instruments which require high ROX for signal correction, such as ABI Prism7000/7300/7700/7900，Eppendorf，ABI Step One/Step One Plus etc
Features
This product uses a new high-performance hot-start enzyme (Golden Star Taq DNA Polymerase) and a unique PCR buffer system. This product significantly improves the PCR amplification efficiency, sensitivity, and specificity.
Notes
- Mix gently before use, avoid foaming, and use after brief centrifugation.
- This product contains SYBR Green I fluorescent dye and ROX dye. Avoid strong light irradiation during the storage.
- Avoid repeated freezing and thawing. Repeated freezing and thawing may comprise product performance.
- This product cannot be used for qPCR using probes.
