Manual

Product Number: PCM33

Shipping and Storage

-20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing.

Kit Components

Product Introduction

The 2×Ultra Sybr qPCR Mix is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains Golden Star Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg²⁺ . The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The Golden Star Taq DNA Polymerase in the mixture is a chemically-modified, new efficient hot-start enzyme that does not have polymerase activity at room temperature which prevents non-specific amplification efficiently, and it is activated by incubation at 95°C for 10 minutes. The combination of a unique PCR buffer system and a hot-start enzyme effectively inhibits non-specific PCR amplification and significantly increases the amplification efficiency of PCR.

This product is suitable for fluorescent qPCR instruments that do not require ROX as a calibration dye, such as Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, and CFX96.

Features

1. This product uses a new high-performance hot start enzyme (Golden Star Taq DNA Polymerase) and a unique PCR buffer system. This product significantly improves the PCR amplification efficiency and has high sensitivity and specificity.
2. This product is suitable for quantitative PCR detection and can accurately quantify and detect the target gene.

Note

1. Mix gently before use, avoid foaming, and use after brief centrifugation.
2. This product contains SYBR Green I fluorescent dye. Avoid strong light irradiation when storing this product or preparing PCR reaction solution.
3. Avoid repeated freezing and thawing of this product. Repeated freezing and thawing may comprise product performance.
4. This product cannot be used for qPCR using probes.
5. When preparing the reaction solution, use new or noncontaminated tips and centrifuge tubes to prevent contamination.

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