Manual

Product Number: PCM27

Shipping and Storage

-20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing.

Components

Description

The 2×Quick Probe Mixture a premixed system specifically designed for real-time fluorescence quantitative PCR using probe methods (TaqMan, Molecular Beacon, etc.), and the concentration is 2×. It contains Quick Taq DNA Polymerase、PCR Buffer、dNTPs、Mg²⁺ etc. The operation is simple and convenient.Mainly used for detecting genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.This product contains Fast Taq DNA Polymerase, which can effectively reduce non-specific amplification caused by non-specific binding of primers and templates or primer dimers at room temperature. The activation of the enzyme only requires incubation at 95℃ for 30 seconds.The entire PCR reaction process can save about 40 minutes compared to ordinary reactions, greatly shortening the reaction time of PCR. The combination of a unique PCR buffer system and rapid hot start enzymes effectively inhibits the production of non-specific products and significantly improves the amplification efficiency of PCR. The fluorescence signal is stronger, the sensitivity is higher, and the linear range is wider. This product has a wide range of applications and can be used for both regular and rapid quantitative PCR programs.

ROX dye is used to correct the fluorescence signal errors generated from hole to hole in quantitative PCR instrument. It is generally used in ABI, Stratagene and other companies' Real Time PCR amplifiers. The excitation optical system varies from instrument to instrument, so the concentration of ROX dye must be matched with the corresponding fluorescence quantitative PCR instrument.

Instruments without ROX correction:Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, CFX96, etc.

Instruments requiring Low ROX correction:ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000, etc.

Instruments requiring High ROX calibration:ABI Prism7000/7300/770/7900, Eppendorf, ABI Step One/Step One Plus, etc.

Notes

1. Mix gently before use, avoid foaming, and use after brief centrifugation.
2. Avoid repeated freeze-thaw of this product, as repeated freeze-thaw may cause a decrease in product performance. This product can be stored for a long time at -20℃, away from light. If frequent use is required in the short term, it can be stored at 2-8℃.