Manual

Product Number: PCM11B

Shipping and Storage

-20 ℃. For frequent use, it can be stored at 2-8℃.

Components

Note: 2×pfu PCR Super MasterMix, with blue dyecontains Pfu DNA Polymerase, 3mM MgCl₂ and 400µM each dNTP。

Description

This product is a premixed system composed of Pfu DNA Polymerase, Mg²⁺, dNTPs, PCR stabilizers and enhancers, with a concentration of 2 ×。 Pfu DNA Polymerase has 5 '-3' DNA polymerase activity and 3 '-5' exonuclease activity, thus possessing error correction ability during DNA amplification.Compared with Taq DNA Polymerase, it has high fidelity (6-8 times that of Taq enzyme) and better thermal stability. The pre prepared PCR mixture makes the operation simpler and faster, minimizing human error and pollution. The original MasterMix formula makes the entire reaction system very stable and has good repeatability. This product has been added with a dye (blue) and can be directly subjected to electrophoresis detection after the reaction is completed. The Pfu DNA polymerase contained in this product has the characteristics of low mismatch rate and high temperature resistance, making it suitable for gene cloning, gene site-specific mutations, SNP, and terminal complement reactions.

Quality Control

After testing, there was no exogenous nuclease activity; PCR method for detecting non host residual DNA; it can effectively amplify single copy genes from multiple genomes; Storage at 2-8 ℃ for three months showed no significant change in activity.

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