Manual

Product Number: PCM10B

Storage condition

-20℃

Component

Description

This product is a premixed system composed of a new and efficient fast DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2×.This product is an innovative new type of fast DNA polymerase with extremely high amplification speed and stability. The extension speed can reach 5 s/kb, and the PCR can be completed in as little as 15 minutes. Long fragments (greater than 3 kb) or complex templates can use an extension speed of 10-30 s/kb or a large number of cycles. The original MasterMix formula makes the entire reaction system very stable, while complex templates can also be effectively amplified, with over 98% of PCR amplification being successful at once. When in use, only DNA templates and primers need to be added, and sufficient water can be added to react, which can minimize human error, reduce pollution, and save time.

This product has been added with a dye (blue) and can be directly subjected to electrophoresis detection after the reaction is completed. The amplified PCR product has an "A" base attached to its 3 'end, making it suitable for T/A cloning and suitable for seamless cloning kits, T4 linking kits, and receptive products.

This product is mainly suitable for experiments such as ultra fast PCR, complex templates, complex secondary structures, gene cloning with high fidelity requirements, and large-scale gene detection.

Quality control

After testing, there was no exogenous nuclease activity; PCR method for detecting non host residual DNA; Can effectively amplify single copy genes from multiple genomes.