Manual

Product Number: PCM56B

Shipping and Storage

-20℃. For frequent use, it can be stored at 2-8℃

Components

Note: The 2×HotStar Best MasterMix (Dye) contains HotStar Best DNA Polymerase,3.4mM MgCl₂ and 400µM each dNTP.

Description

This product is a premixed system composed of HotStar Best DNA Polymerase, Mg²⁺, dNTPs, PCR stabilizers and enhancers, with a concentration of 2×,It has the advantages of simple and fast operation, high sensitivity, strong specificity, and good stability, which can minimize human error and pollution to the greatest extent. The HotStar Best DNA Polymerase contained in this product is a chemically modified hot start high fidelity polymerase.This polymerase has 5'-3' DNA polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonuclease activity. Under ordinary PCR conditions, compared with GoldenStar Taq DNA polymerase, it has excellent performance of high amplification efficiency and low mismatch rate.The chemically modified enzyme has no polymerase activity at room temperature, effectively avoiding non-specific amplification caused by non-specific binding of primers and templates or primer dimers at room temperature. The activation of the enzyme must be incubated at 95℃ for 10 minutes, which can be integrated into existing PCR thermal cycling programs.The optimized buffer system maximizes the effectiveness of the enzyme, achieving high fidelity, specificity, amplification efficiency, and sensitivity for the target fragment.This product has been added with a dye (blue) and can be directly subjected to electrophoresis detection after the reaction is completed.Most of the PCR products obtained by amplification have an "A" base attached to the 3'end, so they can be directly used for T/A cloning. Suitable for routine PCR reactions and gene cloning experiments with high fidelity requirements.

Quality Control

After testing, there was no exogenous nuclease activity; PCR method for detecting non host residual DNA; Can effectively amplify single copy genes from multiple genomes; Storage at 2-8 ℃ for three months showed no significant change in activity.