SNP Genotyping qPCR MIX, UNG
2025-01-14
Bst 3.0 Enzyme Mix
2025-01-14another name: Thermo Start Bst DNA Polymerase
Product Number: BS02
Shipping and Storage
-20°C.
Components
| Component | BS02 1600U |
| WellStart II BST DNA polymerase(8U/μL) | 200μL |
| 10×WellStart II BST Reaction Buffer | 1.5mL |
| 100mM MgSO4Solution | 1.5mL |
Description
WellStart II BST DNA polymerase is a recombinant enzyme expressed and purified by Escherichia coli. Its gene is derived from Bacillus stearothermophilus and has undergone partial point mutations based on the original sequence.This protein has stronger 5'→ 3' DNA polymerase activity, chain displacement activity, reverse transcriptase activity, and no 5'→ 3' exonuclease activity.Applied to DNA/RNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), etc.
Unit definition
The amount of enzyme required to add 10nmol of deoxynucleotides to acidic insoluble substances within 30 minutes at 65℃ is defined as 1 active unit (U).
Heat Inactivation
Incubate at 80°C for 5 minutes before inactivation.
Quality Control
After multiple column purification, the purity was detected by SDS-PAGE to be greater than 98%; No exogenous nuclease activity was detected.
Test
COVID-19 N gene was used as a target in the RT-LAMP reaction.
No activity at room temperature. Will be activated at 60°C.
