Vaccinia Capping System
2021-12-22
mRNA Cap 2'-O-Methyltransferase
2021-12-22Product Number: PC01
Shipping and Storage
-20°C
Components
| Component | PC01 500U | PC01 2500U | PC01 10000U |
| Taq DNA Polymerase,5 U/μL | 100μL | 5×100μL | 2×1mL |
| 10×PCR Buffer | 1.8mL | 5×1.8mL | 8×5mL |
Note: The 10×PCR Buffer of this product contains 15 mM magnesium ions.
Description
The Taq DNA Polymerase is a purified recombinant enzyme expressed by E. coli. The gene is derived from Thermus Aquaticus polymerase. The molecular weight of the protein is 94 kDa, and it has 5 '→3' DNA polymerase activity and 5 '→3' exonuclease activity, but no 3 '→5' exonuclease activity. The elongation rate of the enzyme was 2 KB /min, and the length of 5 KB fragment could be amplified. The amplified PCR product has an "A" base attached to its 3 'end, so it can be directly used for T/A cloning. This product has the characteristics of fast extension with high speed and high amplification efficiency, mainly suitable for PCR amplification of DNA fragments, DNA sequence determination and other experiments.
Activity definition
Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acidic insoluble material was defined as 1 active unit (U) at 74°C for 30 minutes.
Quality control
The purity is grater than 99% after column purification by SDS-PAGE. No exogenous nuclease activity was detected. PCR was used to detect residual DNA without host. It can effectively amplify single copy gene in human genome. There was no obvious activity change after one month storage at room temperature.
