Manual

Product Number: PC77

Shipping and Storage

-20°C.

Components

Note: The 10×PCR Buffer of this product contains 15mM magnesium ions.

Description

QuickPro DNA Polymerase is a mixture of Taq monoclonal antibody and Taq DNA Polymerase with high amplification and fidelity. It is suitable for HOT Start PCR. When using QuickPro DNA Polymerase in PCR amplification, the Taq enzyme antibody inhibited the activity of DNA polymerase before denaturation at high temperature, which effectively inhibited the non-specific annealing of primers and the non-specific amplification caused by primer dimers at low temperatures. The Taq enzyme antibody was denatured in the initial DNA denaturation step of PCR reaction, and the DNA polymerase activity gradually restored to achieve the thermal activation effect. This product does not require special inactivation of Taq enzyme antibodies and can be used under conventional PCR reaction conditions.

QuickPro DNA Polymerase has 5'→3' DNA polymerase activity, 5'→3' exonuclease activity and 3'→5' exonuclease activity. Compared with Taq DNA Polymerase, QuickPro DNA Polymerase has high amplification efficiency and low mismatch rate, which can efficiently amplify DNA fragments. This product can be used directly for T/A cloning because the 3' end of the amplified PCR product has an "A" base. This product is suitable for routine PCR reaction and gene cloning for high fidelity reaction.

Unit definition

After column purification, the purity was more than 99% according to SDS-PAGE. No exogenous nuclease activity was detected. No host residual DNA was detected by PCR. Effectively amplify single-copy genes in the human genome.