Deoxyribonuclease I, DNase I lyophilized powder
2021-12-22
HotStar TaqMan Probe qPCR Mix with low rox
2021-12-22Product Number: PC0301
Shipping and Storage
-20°C
Components
| Component | PC0301 100U | PC0301 500U |
| Pfu DNA Polymerase,2U/μL | 50μL | 250μL |
| 2×Pfu DNA Polymerase Buffer | 2×1.25mL | 7×1.8mL |
| dNTP Mix,10mM each | 150μL | 750μL |
Description
Pfu DNA Polymerase is a fast and high-fidelity DNA Polymerase with high amplification efficiency. The Polymerase possesses 5 '-3' DNA Polymerase activity and 3 '-5' exonuclease activity. The enzyme was modified by other high-fidelity enzymes with strong amplification ability, fast amplification speed and high fidelity, which overcame the defects of ordinary Pfu enzyme such as poor amplification ability, low yield and slow amplification speed, and greatly shortened the reaction time. This product can be used for long fragment amplification and the expansion of other complex templates. The 3 'end of the amplified PCR product does not contain "A" base, and can be directly cloned in the flat terminal vector. If T/A cloning is needed, "A" should be added to the end of the PCR product for cloning. This product is suitable for gene cloning, gene point mutation, SNP amplification experiments.
Unit definition
Absolute ethanol, 10mM PBS (PH7.4)
Quality Control
After multiple column purification, the purity was more than 98% by SDS-PAGE. No exogenous nuclease activity was detected. After one month at room temperature, there was no obvious change of activity.
