Manual

Description

The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.

Taq DNA polymerase: Has the 5' to 3' DNA polymerase activity and 5' to 3' exonuclease activity without 3' - 5' exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3' end. The PCR product can be cloned in TA vector.

Applications

• Hot-start PCR amplification
• Specific amplification of complex cDNA and genomic template
• Amplification from low copy number DNA template
• Real-Time PCR
• Multiple PCR
• Generation of PCR products for TA cloning, Gene microarray analysis and SNP test

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of HotStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Concentration: 5 u/μl

Package: Bulk

Storage: Store at -20°C

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