Manual

Product Number: PC54

Shipping and Storage

-20°C

Components

Note: The 5×HotStar PCR Buffer of this product contains 8.5 mM magnesium ions.

Description

This product is a chemically modified hot-start high-fidelity polymerase. The polymerase has 5'-3' DNA polymerase activity, 5'-3' exonuclease activity and 3'-5' exonuclease activity. Under normal PCR conditions, compared with Golden Star Taq DNA Polymerase, it has higher amplification efficiency excellent performance with high and low mismatch rate. The chemically modified enzyme has no polymerase activity at room temperature, which can effectively avoid non-specific amplification caused by the non-specific binding of primer and template or primer dimer under normal temperature conditions. The activation of the enzyme must be incubated at 95°C for 10 minutes his which can be integrated into existing PCR thermal cycling programs. The optimized buffer system maximizes the effect of the enzyme to achieve high fidelity, high specificity, high amplification efficiency, and high sensitivity amplification of the target fragment. Most of the PCR products amplified with this product have an "A" base attached to the 3' end, so they can be directly used for T/A cloning. This product is used in conventional PCR, RT-PCR and multiplex PCR,especially for PCR with high requirements on specificity, fidelity and amplification efficiency

Active Definition

Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10nmol of deoxynucleotides into acid insoluble substances within 30 minutes at 74°C was defined as 1 activity unit (U).

Quality Control

After several column purifications, the purity of the SDS-PAGE test is more than 99%; no exogenous nuclease activity is detected; no host residual DNA is detected by PCR method; it can effectively amplify single-copy genes in the human genome; it can be stored at room temperature for one month , no significant activity changes.