Manual

Product Number: PC18

Shipping and Storage

Storage at -20℃.

Component

Description

The Klenow Fragment, also known as the Klenow fragment, is a large fragment of Escherichia coli DNA polymerase I (E. coli DNA polymerase I). It retains the 5’→3′ polymerase activity and 3’→5′ exonuclease activity of DNA polymerase I but lacks the complete 5’→3′ exonuclease activity of the full Klenow enzyme. The 3’→5′ exonuclease activity of the Klenow Fragment ensures the fidelity (proofreading) of DNA synthesis.

Application

Fill in at the 5 ‘overhang end of double stranded DNA; Flattening (also known as flattening) the 3 ‘overhang of double stranded DNA; 5 ‘protruding end mark; Random primer method for DNA labeling; Sanger deoxygenation method for DNA sequencing; The synthesis of the second strand of cDNA or site directed mutagenesis reaction.

Features

For adhesive ends with 5 ‘or 3’ protrusions, it can catalyze the generation of flat ends for subsequent flat end connections.