Manual

Product Number: BS04

Shipping and Storage

-20°C.

Components

Component	BS04	BS04
Bsu DNA Polymerase	200U	1000U
10×Reaction Buffer	0.2ml	1.0ml

Description

Bsu DNA polymerase is a full-length protein of DNA polymerase A from Bacillus subtilis, which has a 5 '→ 3' exonuclease domain. However, this polymerase naturally lacks 3 '→ 5' exonuclease activity.

Our company's Bsu DNA polymerase is a recombinant protein expressed and purified through multiple steps.

Concentration

5U/μl

Features

1. Thermal deactivation: 75℃, 20min
2. Has certain incision translation activity

Application

1. RPA isothermal nucleic acid amplification reaction
2. Random primer labeling
3. Synthesis of the second strand of cDNA
4. Adding Tail to a Single dA

Unit definition

One unit is defined as the amount of enzyme that blends 10 nmol dNTP into an acid insoluble substance within 30 minutes at 37°C

Activity determination conditions

10×Reaction Buffer,37℃。

Storage buffer

50 mM Tris-HCl,50 mM KCl,1 mM DTT,0.1mM EDTA,50% Glycerol,pH 7.5,25°C。

Quality control

Relevant tests have shown that there is no contamination of exogenous endonuclease, exonuclease, or RNase. PCR method for detecting residual DNA without host.