HotStar TaqMan Probe qPCR Mix with low rox
2021-12-22
NTP set 100mM solution
2021-12-22Product Number: PC04
Shipping and Storage
-20℃。
Components
| Component | PC04M 2500U |
| mTaq DNA Polymerase,5 U/μl | 5×100μl |
| mTaq PCR Buffer,10× | 5×1.8ml |
Note: mTaq PCR Buffer contains 30mM MgCl2.
Description
mTaq DNA Polymerase is a new type of DNA Polymerase which is modified by deletion of an amino acid segment in the N terminal of Taq DNA polymerase and mutation. Modified to tolerate inhibitors present in whole blood, the product can directly amplify DNA in whole blood samples of humans and mice without prior genome extraction and purification. This product can be used directly for T/A cloning because the 3' end of the amplified PCR product has an "A" base.
Quality control
After column purification, the purity was more than 99% according to SDS-PAGE. No exogenous nuclease activity was detected. No host residual DNA was detected by PCR. Can effectively amplify single copy genes in human genome; Stored at room temperature for one week, no obvious change in activity.
