Manual

Description

The mTaq DNA Polymerase is the mutant of the general Taq DNA Polymerase. mTaq has the better anti inhibitor capacity and high amplification efficiency, can directly use for amplification of blood interstitial fluid and other crude template. The extending speed is 1-2 kb/min. The PCR product with an "A" on 3' end, can be cloned in TA vector.

Concentration: 5 u/µl

Package: Bulk

Storage: Store at -20°C

Unit Definition

One unit of mTaq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Features

• Can directly use for amplification frozen or fresh blood, template do not need pretreatment or purification.
• Directly amplify the template of dried blood and interstitial fluid.
• Amplify the DNA from the crude template such as feces and soil extractive.
• The amplification speed is twice of the general Taq DNA Polymerase.

Quality control

• Functional absence of double and single-stranded endonuclease activity;
• Purity>99% test by SDS gel electrophoresis;
• Each lot of mTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Application

• Crude template DNA amplification.
• Genotyping
• complex DNA template amplification.
• High throughput genetic analysis.

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