Description

N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

Product Name

N1-Me-Pseudo-UTP, 100mM Solution

(N1-Me-Pseudouridine-5'-Triphosphate trisodium salt solution)

CAS No.

1428903-59-6

Molecular Formula

C₁₀H₁₄N₂Na₃O₁₅P₃

Molecular Weight

564.11

Molar absorptivity

8500 (λ=271nm)

Specification

Appearance	Clear colorless solution
Purity(by HPLC)	≥99.0%
λmax	272nm±2nm
Concentration	100mM±3
31P NMR(D2O)	To conform structure
1H NMR(D2O)	To conform structure
pH(in buffer solution)	7.0±0.1

Storage Recommendation

Please store in -20°C. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

Quality Control

1. The concentration is verified by Uv-vis spectrophotometry.

2. This lot of N1-Me-Pseudo-UTP is free of DNase and RNase contamination.

3. The purity of this preparation is determined by HPLC.

Order

N5331

N1 methyl Pseudo UTP 100mM solution

100mM N1 methyl Pseudo UTP, Purity>99% (HPLC), suitable for mRNA modification