CY3-D5331

dNTP

Cy3-dUTP 1mM Sodium solution

Description

Cy3-dUTP can be used for direct enzymatic labeling of DNA, cDNA by PCR with Taq polymerase, Nick Translation with DNAse I, DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dUTP can replace TTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and MMLV) and terminal transferase. The synthesized dye-labeled DNA, cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dUTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dUTP, dTTP ratio for PCR and Nick Translation: 30-50% Cy3-dUTP, 50% dTTP.

Features

• High sensitivity.
• Low nonspecific binding.
• High photostability.
• High water solubility.
• pH insensitive.

Order